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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00004774-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00004774-M02, RRID:AB_875735
- Product name
- NFIA monoclonal antibody (M02), clone 1E11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant NFIA.
- Antigen sequence
MYSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQA
RKRKYFKKHEKRMSKEEERAVKDELLSEKPEVKQK
WASRLLAKLRKDIRPEYREDFVLTVTGKKPPCCVL
SNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGI
PLESTDGERLVKSPQCSNPGLCVQPHHIGVSVKEL
DLYLAYFVHAADSSQSESPSQPSDADIKDQPENGH
LGFQDSFVTSGVFSVTELVRVSQTPIAAGTGPNFS
LSDLESSSYYSMSPGAMRRSLPSTSSTSSTKRLKS
VEDEMDSPGEEPFYTGQGRSPGSGSQSSGWHEVEP
GMPSPTTLKKSEKSGFSSPSPSQTSSLGTAFTQHH
RPVITGPRASPHATPSTLHFPTSPIIQQPGPYFSH
PAIRYHPQETLKEFVQLVCPDAGQQAGQVGFLNPN
GSSQGKVHNPFLPTPMLPPPPPPPMARPVPLPVPD
TKPPTTSTEGGAASPTSPILVPGIKVAASHHPPDR
PPDPFSTL- Isotype
- IgG
- Antibody clone number
- 1E11
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references The interplay between the master transcription factor PU.1 and miR-424 regulates human monocyte/macrophage differentiation.
Rosa A, Ballarino M, Sorrentino A, Sthandier O, De Angelis FG, Marchioni M, Masella B, Guarini A, Fatica A, Peschle C, Bozzoni I
Proceedings of the National Academy of Sciences of the United States of America 2007 Dec 11;104(50):19849-54
Proceedings of the National Academy of Sciences of the United States of America 2007 Dec 11;104(50):19849-54
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of NFIA expression in transfected 293T cell line by NFIA monoclonal antibody (M02), clone 1E11.Lane 1: NFIA transfected lysate (Predicted MW: 54.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged NFIA is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to NFIA on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
