Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-52252 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NFIA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LKSVEDEMDS PGEEPFYTGQ GRSPGSGSQS SGWHEVEPGM PSPTTLKKSE KSGFSSPSPS QTSSLGTAFT QHHRPVITGP RASPHATPST LHFPTSPIIQ Q
- Concentration
- 0.1 mg/mL
Submitted references The miR-223/nuclear factor I-A axis regulates inflammation and cellular functions in intestinal tissues with necrotizing enterocolitis.
Wu YZ, Chan KYY, Leung KT, Lam HS, Tam YH, Lee KH, Li K, Ng PC
FEBS open bio 2021 Jul;11(7):1907-1920
FEBS open bio 2021 Jul;11(7):1907-1920
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NFIA in mouse cell line NIH-3T3 and rat cell line NBT-II using a NFIA Polyclonal Antibody (Product # PA5-52252).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NFIA in human cell lines HeLa and Caco-2 using a NFIA Polyclonal Antibody (Product # PA5-52252). Corresponding NFIA RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NFIA in human cell line U-251 MG shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a NFIA Polyclonal Antibody (Product # PA5-52252).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NFIA in human colon using NFIA Polyclonal Antibody (Product # PA5-52252).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NFIA in human thyroid gland using a NFIA Polyclonal Antibody (Product # PA5-52252) shows nuclear positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NFIA in human cerebral cortex using NFIA Polyclonal Antibody (Product # PA5-52252).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NFIA in human testis using NFIA Polyclonal Antibody (Product # PA5-52252).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NFIA in human lymph node using NFIA Polyclonal Antibody (Product # PA5-52252).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 Relative expression level of miR-223 and NFIA in Caco-2 cells upon overexpression of mimic miR-223. (A) Relative expression of miR-223 to U6 and (B) NFIA to GAPDH in Caco-2 cells after transfection with mimic-CTL and mimic miR-223 by qPCR ( n = 6). Expressions of miR-223 and NFIA were normalized with U6 and GAPDH, respectively. (C, D) The protein level of NFIA was measured by western blot analysis after overexpression of mimic miR-223. Statistical analysis was performed by paired t -test, two-sided. The data were presented as mean +- SEM.
