NBP1-81406

antibody from Novus Biologicals

Targeting: NFIA KIAA1439, NFI-L

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-81406

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-81406, RRID:AB_11012375

Product name

Rabbit Polyclonal NFIA Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human NFIA antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

PAX6 does not regulate Nfia and Nfib expression during neocortical development.

Bunt J, Lim JW, Zhao L, Mason S, Richards LJ
Scientific reports 2015 May 29;5:10668

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NFIA Antibody [NBP1-81406] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NFIA Antibody [NBP1-81406] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NFIA Antibody [NBP1-81406] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NFIA Antibody [NBP1-81406] - Analysis in human cell line HeLa and human cell line CACO-2.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81406] - Staining of human fallopian tube shows moderate nuclear positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81406] - Staining of human prostate shows strong nuclear positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81406] - Staining of human thyroid gland shows strong nuclear positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81406] - Staining of human tonsil shows moderate nuclear positivity in lymphoid cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81406] - Staining in human thyroid gland and tonsil tissues . Corresponding NFIA RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81406] - Staining of human lymph node.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81406] - Staining of human testis.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81406] - Staining of human colon.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81406] - Staining of human cerebral cortex.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81406] - Staining of human testis shows strong nuclear positivity in Leydig cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81406] - Staining of human gastrointestinal shows strong nuclear positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81406] - Staining of human lymphoid tissues shows strong nuclear positivity in non-germinal center cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81406] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81406] - Staining of human cerebral cortex, gastrointestinal, lymphoid tissues and testis using Anti-NFIA antibody (A) NBP1-81406 shows similar protein distribution across tissues to independent antibody NBP1-81407 (B).