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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- TA501648 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501648, RRID:AB_11126243
- Product name
- PRKD2 (Protein Kinase D2) mouse monoclonal antibody, clone OTI5A3 (formerly 5A3)
- Antibody type
- Monoclonal
- Description
- PRKD2 (Protein Kinase D2) mouse monoclonal antibody, clone OTI5A3 (formerly 5A3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PRKD2
- Isotype
- IgG
- Antibody clone number
- OTI5A3
- Vial size
- 100 µl
- Concentration
- 0.97 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKD2 (RC215335, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKD2.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-PRKD2 mouse monoclonal antibody (TA501648) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKD2(RC215335).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PRKD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501648)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-PRKD2 antibody(TA501648),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC215335 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PRKD2 antibody(TA501648), and then analyzed by flow cytometry.
- Validation comment
- FC
