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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- RQ6587 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- TBP-1 Antibody / PSMC3
- Antibody type
- Monoclonal
- Description
- Antigen affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Antibody clone number
- 4D3
- Vial size
- 100 µg
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the PSMC3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HEK293, 2) human HeLa, 3) human HepG2, 4) human PC-3, 5) rat stomach, 6) rat kidney, 7) mouse stomach and 8) mouse kidney tissue lysate with PSMC3 antibody. Predicted molecular weight ~50 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human MCF7 cells with PSMC3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human colonic adenocarcinoma tissue with PSMC3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human liver cancer tissue with PSMC3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U937 cells with PSMC3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PSMC3 antibody.
