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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- BML-PW8770-0025 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#BML-PW8770-0025, RRID:AB_2052361
- Product name
- Proteasome 19S Rpt5/S6a subunit monoclonal antibody (TBP1-19)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full length protein
- Description
- Partially purified ascites.
- Reactivity
- Human, Mouse, Rat, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- TBP1-19
- Vial size
- 25 μl
- Storage
- -20°C
Submitted references Skeletal muscle myotubes in severe obesity exhibit altered ubiquitin-proteasome and autophagic/lysosomal proteolytic flux.
Bortezomib Amplifies Effect on Intracellular Proteasomes by Changing Proteasome Structure.
Bollinger LM, Powell JJ, Houmard JA, Witczak CA, Brault JJ
Obesity (Silver Spring, Md.) 2015 Jun;23(6):1185-93
Obesity (Silver Spring, Md.) 2015 Jun;23(6):1185-93
Bortezomib Amplifies Effect on Intracellular Proteasomes by Changing Proteasome Structure.
Pitcher DS, de Mattos-Shipley K, Tzortzis K, Auner HW, Karadimitris A, Kleijnen MF
EBioMedicine 2015 Jul;2(7):642-8
EBioMedicine 2015 Jul;2(7):642-8
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Supportive validation
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- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis: Luminograph of rabbit 26S proteasome preparation after SDS PAGE followed by blotting onto PVDF membrane and probing with antibody BML-PW8770. Antibody dilution 1:1000 and 1:2500 using ECL procedure (1 min exposure).
