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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- AP2511b - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP2511B, RRID:AB_2182919
- Product name
- SAE1 (AOS1) Antibody (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- The AOS1 C-term Pab (Cat. #AP2511b) is used in Western blot to detect AOS1 in mouse heart tissue lysate.
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of AOS1 (arrow) using rabbit polyclonal AOS1 Antibody (C-term) (Cat.#AP2511b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOS1 gene (Lane 2) (Origene Technologies).
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- AOS1 Antibody (V315) (Cat. #AP2511b) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the AOS1 antibody detected the AOS1 protein (arrow).
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100
