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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00010055-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00010055-M01, RRID:AB_539700
- Product name
- Mouse Monoclonal SUMO Activating Enzyme E1 (SAE1) Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SAE1 - SUMO-1 activating enzyme subunit 1
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Clinical characteristics of anti-SAE antibodies in Chinese patients with dermatomyositis in comparison with different patient cohorts.
Autoantibodies to small ubiquitin-like modifier activating enzymes in Japanese patients with dermatomyositis: comparison with a UK Caucasian cohort.
Ge Y, Lu X, Shu X, Peng Q, Wang G
Scientific reports 2017 Mar 15;7(1):188
Scientific reports 2017 Mar 15;7(1):188
Autoantibodies to small ubiquitin-like modifier activating enzymes in Japanese patients with dermatomyositis: comparison with a UK Caucasian cohort.
Fujimoto M, Matsushita T, Hamaguchi Y, Kaji K, Asano Y, Ogawa F, Yamaoka T, Fujikawa K, Tsukada T, Sato K, Echigo T, Hasegawa M, Takehara K
Annals of the rheumatic diseases 2013 Jan;72(1):151-3
Annals of the rheumatic diseases 2013 Jan;72(1):151-3
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5) [H00010055-M01] - SAE1 monoclonal antibody (M01), clone 1G4-1G5 Analysis of SAE1 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5) [H00010055-M01] - Detection limit for recombinant GST tagged SAE1 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5) [H00010055-M01] - Analysis of monoclonal antibody to SAE1 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5) [H00010055-M01] - Analysis of monoclonal antibody to SAE1 on formalin-fixed paraffin-embedded human adrenal gland. Antibody concentration 3 ug/ml.
