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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-22297 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SAE1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, H1299, mouse brain, Rat2.
- Concentration
- 0.72 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SAE1 Polyclonal Antibody (Product # PA5-22297). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: H1299. 10% SDS PAGE. SAE1 Polyclonal Antibody (Product # PA5-22297) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SAE1 Polyclonal Antibody (Product # PA5-22297). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. SAE1 Polyclonal Antibody (Product # PA5-22297) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SAE1 Polyclonal Antibody detects SAE1 protein by Western blot analysis. A. 30 µg Rat2 whole cell lysate/extract.10 % SDS-PAGE. SAE1 Polyclonal Antibody (Product # PA5-22297) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SAE1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: SAE1 Polyclonal Antibody (Product # PA5-22297) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using SAE1 (Product # PA5-22297) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
