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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP51047PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP51047PU-N, RRID:AB_11147403
- Product name
- anti Carboxypeptidase B (N-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 16-45 amino acids from the N-terminal region of Human CPB1
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Carboxypeptidase B Antibody (N-term) Cat.-No AP51047PU-N in K562,Hela cell line lysates (35ug/lane).This demonstrates the CPB1 antibody detected the CPB1 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of Carboxypeptidase BÂ Antibody (N-term) Cat.-No AP51047PU-NÂ with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin fixed and paraffin embedded human pancreas tissue stained with Carboxypeptidase B Antibody (N-term) Cat.-No AP51047PU-Nfollowed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of CPB1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells using Carboxypeptidase B Antibody (N-term) Cat.-No AP51047PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
