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Learn14286-1-AP
antibody from Invitrogen Antibodies
Targeting: HLTF
HIP116A, HLTF1, RNF80, SMARCA3, SNF2L3
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- 14286-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HLTF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSWMFKRDP VWKYLQTVQY GVHGNFPRLS YPTFFPRFEF QDVIPPDDFL TSDEEVDSVL FGSLRGHVVG LRYYTGVVNN NEMVALQRDP NNPYDKNAIK VNNVNGNQVG HLKKELAGAL AYIMDNKLAQ IEGVVPFGAN NAFTMPLHMT FWGKEENRKA VSDQLKKHGF KLGPAPKTLG FNLESGWGSG RAGPSYSMPV HAAVQMTTEQ LKTEFDKLFE DLKEDDKTHE MEPAEAIETP LLPHQKQALA WMVSRENSKE LPPFWEQRND LYYNTITNFS EKDRPENVHG GILADDMGLG KTLTAIAVIL TNFHDGRPLP IERVKKNLLK KEYNVNDDSM KLGGNNTSEK ADGLS (1-354 aa encoded by BC044659)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 14286-1-AP (HLTF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 14286-1-AP (HLTF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 14286-1-AP (HLTF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-HLTF (IP:14286-1-AP, 4ug; Detection:14286-1-AP 1:500) with HeLa cells lysate 2400ug.
