RQ8132

antibody from NSJ Bioreagents

Targeting: NFRKB DKFZp547B2013, INO80G

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ8132

Provider

NSJ Bioreagents

Product name

NFRKB Antibody / Nuclear factor related to kappa-B-binding protein

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human

Host

Rabbit

Vial size

100 µg

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the NFRKB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HEL, 2) RT-4, 3) Caco-2 and 4) HaCaT cell lysate with NFRKB antibody. Predicted molecular weight ~139 kDa, commonly observed at ~175 kDa and can be observed at ~220 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human A549 cells with NFRKB antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human HEL cells with NFRKB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NFRKB antibody.