HPA052963

antibody from Atlas Antibodies

Targeting: COL17A1 BP180, BPAG2

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA052963

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA052963, RRID:AB_2681999

Product name

Anti-COL17A1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

NLPSHVWSSTLPAGSSMGTYHNNMTTQSSSLLNTN
AYSAGSVFGVPNNMASCSPTLHPGLSTSSSVFGMQ
NNLAPSLTTLSHGTTTTSTA


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human skin and skeletal muscle tissues using Anti-COL17A1 antibody. Corresponding COL17A1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skin shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows low expression as expected.

Sample type

HUMAN