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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP2-42413 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Collagen XVII Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Does not cross-react with mouse and rat skin
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 250 ug
- Concentration
- LYOPH
- Storage
- Lyophilized antibody can be kept at 4C for up to 3 months and should be kept at -20C for long-term storage. To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20C) or for long-term storage (-80C). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Collagen XVII Antibody (NC16a-3) [NBP2-42413] - NC16a-3 detects collagen XVII and its 120kDa shed ectodomain by western blotting with keratinocyte extract (E) and keratinocyte medium (M) respectively.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Collagen XVII Antibody (NC16a-3) [NBP2-42413] - Cryosections of normal human skin and from patients with inherited skin-blistering disorders and absence of collagen XVII or laminin 5 were stained with mAb NC16a-3 and a rabbit anti-laminin 5 antibody.
