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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AM02250PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM02250PU-N, RRID:AB_1622340
- Product name
- anti Collagen type XVII alpha 1 chain (1-452)
- Antibody type
- Monoclonal
- Antigen
- Human type XVII collagen intracellular domain (aa 1-490) GST fusion protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- V58
- Vial size
- 0.1 mg
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot analysis of Human Type XVII Collagen (Cat.-No. AM02250PU-N) specificity. Keratinocytes extracts were separated by SDS-PAGE and immunoblotted with Preimmune (Lane 1) and Immune Mouse serum (Lane 2) as well as with AM02250PU-N (Lane 3). AM02250PU-N detects a specific 180 kDa band.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- V-5-8 Monoclonal Antibody specifically binds to a 180 kDa protein by<strong> Immunoblotting:</strong> Different extracts of Human epidermis (Lanes 1-3) and primary Human keratinocytes (Lanes 4-11) were fractionated by 7.5% SDS-PAGE and immunoblotted with an 100-fold dilution of the Monoclonal antibody V-5-8. <br />Molecular weight markers are depicted to the right.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- V-5-8 Monoclonal Antibody labels the dermal-epidermal junction by Immunofluorescence Microscopy:Â A 1/100 dilution of purified V-5-8 IgG was incubated 30 min at room temprature with Frozen Sections of human skin. After washing, bound IgG was visualized using FITC-conjugated antibodies specific for Mouse (B) IgG and (C) IgG2a (all magnification, x400).
