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LearnPA5-34548
antibody from Invitrogen Antibodies
Targeting: KAT5
cPLA2, ESA1, HTATIP, HTATIP1, PLIP, TIP60, ZC2HC5
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-34548 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TIP60 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is human brain tissue lysate.
- Concentration
- 1 mg/mL
Submitted references The Molecular Effects of Sulforaphane and Capsaicin on Metabolism upon Androgen and Tip60 Activation of Androgen Receptor.
Carrasco-Pozo C, Tan KN, Rodriguez T, Avery VM
International journal of molecular sciences 2019 Oct 29;20(21)
International journal of molecular sciences 2019 Oct 29;20(21)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human brain tissue lysate using a KAT5 polyclonal antibody (Product # PA5-34548) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of KAT5 in human brain tissue lysate with TIP60 Polyclonal Antibody (Product # PA5-34548) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), FaDu (Lane 2), HCT 116 (Lane 3) and MCF-7 (Lane 4). The blot was probed with Anti-TIP60 Polyclonal Antibody (Product # PA5-34548, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 58.6 kDa band corresponding to TIP60 was observed across the cell lines tested (Lanes 1-4).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of TIP60 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with anti-TIP60 Rabbit Polyclonal Antibody (Product # PA5-34548) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing predominantly Nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of KAT5 in rat brain tissue with TIP60 Polyclonal Antibody (Product # PA5-34548) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of KAT5 in rat brain tissue with TIP60 Polyclonal Antibody (Product # PA5-34548) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of KAT5 in rat brain tissue with TIP60 Polyclonal Antibody (Product # PA5-34548) at 2.5 µg/mL.
