RQ6147

antibody from NSJ Bioreagents

Targeting: PAWR par-4, PAR4

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ6147

Provider

NSJ Bioreagents

Product name

Par4 Antibody / Pawr

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 µg

Storage

After reconstitution, the Par4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat kidney, 2) rat stomach, 3) rat liver, 4) mouse liver, 5) mouse pancreas, 6) mouse testis and 7) mouse HEPA1-6 lysate with Par4 antibody. Predicted molecular weight ~45 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE mouse MFC cells (mouse forestomach carcinoma) with Par4 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse brain with Par4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse brain with Par4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat intestine with Par4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of mouse HEPA1-6 cells with Par4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Par4 antibody.