AF6885

antibody from R&D Systems

Targeting: PAWR par-4, PAR4

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF6885

Provider

R&D Systems

Product name

Anti-Human PAWR Antigen Affinity-purified Polyclonal Antibody

Antibody type

Polyclonal

Antigen

E. coli-derived recombinant human PAWR, Arg2-Ala121

Description

Antigen Affinity-purified

Reactivity

Human

Host

Sheep

Antigen sequence

CAD88640


Isotype

IgG

Vial size

100 µg

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

PAWR in LNCaP Human Cell Line. PAWR was detected in immersion fixed LNCaP human prostate cancer cell line using Sheep Anti-Human PAWR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6885) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.