Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX115579 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX115579, RRID:AB_11177791
- Product name
- PRDM4 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Identification of SUMO-2/3-modified proteins associated with mitotic chromosomes.
CubeƱas-Potts C, Srikumar T, Lee C, Osula O, Subramonian D, Zhang XD, Cotter RJ, Raught B, Matunis MJ
Proteomics 2015 Feb;15(4):763-72
Proteomics 2015 Feb;15(4):763-72
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: PC-3 B: U87-MG 7.5% SDS PAGE GTX115579 diluted at 1:5000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PRDM4 antibody detects PRDM4 protein at nucleus by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PRDM4 protein stained by PRDM4 antibody (GTX115579) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PRDM4 antibody detects PRDM4 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PRDM4 protein stained by PRDM4 antibody (GTX115579) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using PRDM4(GTX115579) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded mouse muscle, using PRDM4(GTX115579) antibody at 1:500 dilution.