AM26565AF-N

antibody from Acris Antibodies GmbH

Targeting: LIG1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM26565AF-N

Provider

Acris Antibodies GmbH

Product name

anti DNA ligase 1

Antibody type

Monoclonal

Antigen

Human recombinant DNA Ligase I

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

5H5

Vial size

0.1 mg

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysi s of DNA Li gase I expression in Jurkat cells (1), Raji cell s (2), HL60 cells (3), HeLa cells (4),  WR19L cells (5) and PC12 cells (6) using AM26565AF-N.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunocytochemical detection of DNA Ligase I on acetone fixed HEp-II cells with AM26565AF-N.

Immunoprecipitation

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunoprecipitation of DNA Ligase I from Jurkat cells with AM26565AF-N (1) or mouse IgG1 (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with AM26565AF-N.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemical detection of DNA Ligase I on paraffin embedded section of a human breast cancer with AM26565AF-N.

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Flow cytometric analysis of DNA Ligase I expression in FM3A cells (right) and Jurkat cells (left). Open histogram indicate the reaction of isotypic control to the cells. Shaded histograms indicate the reaction of AM26565AF-N to the cells.