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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [8]
- Other assay [1]
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- Product number
- 18051-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DNA Ligase I Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LVREPLSRR RQLLRENFVE TEGEFVFATS LDTKDIEQIA EFLEQSVKDS CEGLMVKTLD VDATYEIAKR SHNWLKLKKD YLDGVGDTLD LVVIGAYLGR GKRAGRYGGF LLASYDEDSE ELQAICKLGT GFSDEELEEH HQSLKALVLP SPRPYVRIDG AVIPDHWLDP SAVWEVKCAD LSLSPIYPAA RGLVDSDKGI SLRFPRFIRV REDKQPEQAT TSAQVACLYR KQSQIQNQQG EDSGSDPEDT Y (670-919 aa encoded by BC108318)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.14 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 18051-1-AP (DNA Ligase I antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 18051-1-AP (DNA Ligase I antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human spleen using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human spleen using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-DNA Ligase I (IP:18051-1-AP, 4ug; Detection:18051-1-AP 1:500) with HepG2 cells lysate 3200ug.
