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- Antibody Data
- Antigen structure
- References [27]
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- Product number
- NB100-119 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-119, RRID:AB_11028821
- Product name
- Mouse Monoclonal DNA Ligase I Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This is specific for DNA ligase 1.
- Reactivity
- Human, Mouse, Bacteria, Chicken/Avian, Hamster, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Assembly of the base excision repair complex on abasic DNA and role of adenomatous polyposis coli on its functional activity.
DNA polymerase beta-dependent long patch base excision repair in living cells.
Human DNA polymerase beta polymorphism, Arg137Gln, impairs its polymerase activity and interaction with PCNA and the cellular base excision repair capacity.
Low levels of DNA ligases III and IV sufficient for effective NHEJ.
A conserved physical and functional interaction between the cell cycle checkpoint clamp loader and DNA ligase I of eukaryotes.
Base excision repair proteins are required for integrin-mediated suppression of bleomycin-induced DNA breakage in murine lung endothelial cells.
Mechanism of stimulation of human DNA ligase I by the Rad9-rad1-Hus1 checkpoint complex.
DNA ligase III as a candidate component of backup pathways of nonhomologous end joining.
Phenotypic effects of heterozygosity for a BRCA2 mutation.
Histone deacetylase 4 interacts with 53BP1 to mediate the DNA damage response.
BRCA2-dependent and independent formation of RAD51 nuclear foci.
Role of mammalian RAD51L2 (RAD51C) in recombination and genetic stability.
Identification and purification of two distinct complexes containing the five RAD51 paralogs.
The meiosis-specific recombinase hDmc1 forms ring structures and interacts with hRad51.
Polymorphisms in the human DNA ligase I gene (LIG1) including a complex GT repeat.
DNA ligase I selectively affects DNA synthesis by DNA polymerases delta and epsilon suggesting differential functions in DNA replication and repair.
DNA ligase I is recruited to sites of DNA replication by an interaction with proliferating cell nuclear antigen: identification of a common targeting mechanism for the assembly of replication factories.
Structure and function of mammalian DNA ligases.
The BRC repeats in BRCA2 are critical for RAD51 binding and resistance to methyl methanesulfonate treatment.
The BRC repeats in BRCA2 are critical for RAD51 binding and resistance to methyl methanesulfonate treatment.
An interaction between DNA ligase I and proliferating cell nuclear antigen: implications for Okazaki fragment synthesis and joining.
An interaction between DNA ligase I and proliferating cell nuclear antigen: implications for Okazaki fragment synthesis and joining.
Association of BRCA1 with Rad51 in mitotic and meiotic cells.
Association of BRCA1 with Rad51 in mitotic and meiotic cells.
Aberrant DNA repair and DNA replication due to an inherited enzymatic defect in human DNA ligase I.
Aberrant DNA repair and DNA replication due to an inherited enzymatic defect in human DNA ligase I.
Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA.
Jaiswal AS, Narayan S
Biochemistry 2011 Mar 22;50(11):1901-9
Biochemistry 2011 Mar 22;50(11):1901-9
DNA polymerase beta-dependent long patch base excision repair in living cells.
Asagoshi K, Liu Y, Masaoka A, Lan L, Prasad R, Horton JK, Brown AR, Wang XH, Bdour HM, Sobol RW, Taylor JS, Yasui A, Wilson SH
DNA repair 2010 Feb 4;9(2):109-19
DNA repair 2010 Feb 4;9(2):109-19
Human DNA polymerase beta polymorphism, Arg137Gln, impairs its polymerase activity and interaction with PCNA and the cellular base excision repair capacity.
Guo Z, Zheng L, Dai H, Zhou M, Xu H, Shen B
Nucleic acids research 2009 Jun;37(10):3431-41
Nucleic acids research 2009 Jun;37(10):3431-41
Low levels of DNA ligases III and IV sufficient for effective NHEJ.
Windhofer F, Wu W, Iliakis G
Journal of cellular physiology 2007 Nov;213(2):475-83
Journal of cellular physiology 2007 Nov;213(2):475-83
A conserved physical and functional interaction between the cell cycle checkpoint clamp loader and DNA ligase I of eukaryotes.
Song W, Levin DS, Varkey J, Post S, Bermudez VP, Hurwitz J, Tomkinson AE
The Journal of biological chemistry 2007 Aug 3;282(31):22721-30
The Journal of biological chemistry 2007 Aug 3;282(31):22721-30
Base excision repair proteins are required for integrin-mediated suppression of bleomycin-induced DNA breakage in murine lung endothelial cells.
Rose JL, Reeves KC, Likhotvorik RI, Hoyt DG
The Journal of pharmacology and experimental therapeutics 2007 Apr;321(1):318-26
The Journal of pharmacology and experimental therapeutics 2007 Apr;321(1):318-26
Mechanism of stimulation of human DNA ligase I by the Rad9-rad1-Hus1 checkpoint complex.
Wang W, Lindsey-Boltz LA, Sancar A, Bambara RA
The Journal of biological chemistry 2006 Jul 28;281(30):20865-72
The Journal of biological chemistry 2006 Jul 28;281(30):20865-72
DNA ligase III as a candidate component of backup pathways of nonhomologous end joining.
Wang H, Rosidi B, Perrault R, Wang M, Zhang L, Windhofer F, Iliakis G
Cancer research 2005 May 15;65(10):4020-30
Cancer research 2005 May 15;65(10):4020-30
Phenotypic effects of heterozygosity for a BRCA2 mutation.
Warren M, Lord CJ, Masabanda J, Griffin D, Ashworth A
Human molecular genetics 2003 Oct 15;12(20):2645-56
Human molecular genetics 2003 Oct 15;12(20):2645-56
Histone deacetylase 4 interacts with 53BP1 to mediate the DNA damage response.
Kao GD, McKenna WG, Guenther MG, Muschel RJ, Lazar MA, Yen TJ
The Journal of cell biology 2003 Mar 31;160(7):1017-27
The Journal of cell biology 2003 Mar 31;160(7):1017-27
BRCA2-dependent and independent formation of RAD51 nuclear foci.
Tarsounas M, Davies D, West SC
Oncogene 2003 Feb 27;22(8):1115-23
Oncogene 2003 Feb 27;22(8):1115-23
Role of mammalian RAD51L2 (RAD51C) in recombination and genetic stability.
French CA, Masson JY, Griffin CS, O'Regan P, West SC, Thacker J
The Journal of biological chemistry 2002 May 31;277(22):19322-30
The Journal of biological chemistry 2002 May 31;277(22):19322-30
Identification and purification of two distinct complexes containing the five RAD51 paralogs.
Masson JY, Tarsounas MC, Stasiak AZ, Stasiak A, Shah R, McIlwraith MJ, Benson FE, West SC
Genes & development 2001 Dec 15;15(24):3296-307
Genes & development 2001 Dec 15;15(24):3296-307
The meiosis-specific recombinase hDmc1 forms ring structures and interacts with hRad51.
Masson JY, Davies AA, Hajibagheri N, Van Dyck E, Benson FE, Stasiak AZ, Stasiak A, West SC
The EMBO journal 1999 Nov 15;18(22):6552-60
The EMBO journal 1999 Nov 15;18(22):6552-60
Polymorphisms in the human DNA ligase I gene (LIG1) including a complex GT repeat.
Livak KJ, Little WA, Stack SL, Patterson TA
Mutation research 1998 Nov;406(1):1-8
Mutation research 1998 Nov;406(1):1-8
DNA ligase I selectively affects DNA synthesis by DNA polymerases delta and epsilon suggesting differential functions in DNA replication and repair.
Mossi R, Ferrari E, Hübscher U
The Journal of biological chemistry 1998 Jun 5;273(23):14322-30
The Journal of biological chemistry 1998 Jun 5;273(23):14322-30
DNA ligase I is recruited to sites of DNA replication by an interaction with proliferating cell nuclear antigen: identification of a common targeting mechanism for the assembly of replication factories.
Montecucco A, Rossi R, Levin DS, Gary R, Park MS, Motycka TA, Ciarrocchi G, Villa A, Biamonti G, Tomkinson AE
The EMBO journal 1998 Jul 1;17(13):3786-95
The EMBO journal 1998 Jul 1;17(13):3786-95
Structure and function of mammalian DNA ligases.
Tomkinson AE, Mackey ZB
Mutation research 1998 Feb;407(1):1-9
Mutation research 1998 Feb;407(1):1-9
The BRC repeats in BRCA2 are critical for RAD51 binding and resistance to methyl methanesulfonate treatment.
Chen PL, Chen CF, Chen Y, Xiao J, Sharp ZD, Lee WH
Proceedings of the National Academy of Sciences of the United States of America 1998 Apr 28;95(9):5287-92
Proceedings of the National Academy of Sciences of the United States of America 1998 Apr 28;95(9):5287-92
The BRC repeats in BRCA2 are critical for RAD51 binding and resistance to methyl methanesulfonate treatment.
Chen PL, Chen CF, Chen Y, Xiao J, Sharp ZD, Lee WH
Proceedings of the National Academy of Sciences of the United States of America 1998 Apr 28;95(9):5287-92
Proceedings of the National Academy of Sciences of the United States of America 1998 Apr 28;95(9):5287-92
An interaction between DNA ligase I and proliferating cell nuclear antigen: implications for Okazaki fragment synthesis and joining.
Levin DS, Bai W, Yao N, O'Donnell M, Tomkinson AE
Proceedings of the National Academy of Sciences of the United States of America 1997 Nov 25;94(24):12863-8
Proceedings of the National Academy of Sciences of the United States of America 1997 Nov 25;94(24):12863-8
An interaction between DNA ligase I and proliferating cell nuclear antigen: implications for Okazaki fragment synthesis and joining.
Levin DS, Bai W, Yao N, O'Donnell M, Tomkinson AE
Proceedings of the National Academy of Sciences of the United States of America 1997 Nov 25;94(24):12863-8
Proceedings of the National Academy of Sciences of the United States of America 1997 Nov 25;94(24):12863-8
Association of BRCA1 with Rad51 in mitotic and meiotic cells.
Scully R, Chen J, Plug A, Xiao Y, Weaver D, Feunteun J, Ashley T, Livingston DM
Cell 1997 Jan 24;88(2):265-75
Cell 1997 Jan 24;88(2):265-75
Association of BRCA1 with Rad51 in mitotic and meiotic cells.
Scully R, Chen J, Plug A, Xiao Y, Weaver D, Feunteun J, Ashley T, Livingston DM
Cell 1997 Jan 24;88(2):265-75
Cell 1997 Jan 24;88(2):265-75
Aberrant DNA repair and DNA replication due to an inherited enzymatic defect in human DNA ligase I.
Prigent C, Satoh MS, Daly G, Barnes DE, Lindahl T
Molecular and cellular biology 1994 Jan;14(1):310-7
Molecular and cellular biology 1994 Jan;14(1):310-7
Aberrant DNA repair and DNA replication due to an inherited enzymatic defect in human DNA ligase I.
Prigent C, Satoh MS, Daly G, Barnes DE, Lindahl T
Molecular and cellular biology 1994 Jan;14(1):310-7
Molecular and cellular biology 1994 Jan;14(1):310-7
Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA.
Shinohara A, Ogawa H, Matsuda Y, Ushio N, Ikeo K, Ogawa T
Nature genetics 1993 Jul;4(3):239-43
Nature genetics 1993 Jul;4(3):239-43
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNA Ligase I Antibody (10H5) [NB100-119] - Detection of human Ligase I protein in Raji whole cell lysate using NB 100-119.
