H00011153-M02

antibody from Abnova Corporation

Targeting: FICD HIP13, HYPE

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00011153-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00011153-M02, RRID:AB_1576135

Product name

HYPE monoclonal antibody (M02), clone 3F5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant HYPE.

Antigen sequence

MAVTEPKWVSVWSRFLWVTLLSMVLGSLLALLLPL
GAVEEQCLAVLKGLYLLRSKPDRAQHAATKCTSPS
TELSITSRGATLLVAKTKASPAGKLEARAALNQAL
EMKRQGKREKAQKLFMHALKMDPDFVDALTEFGIF
SEEDKDIIQADYLYTRALTISPYHEKALVNRDRTL
PLVEEIDQRYFSIIDSKVKKVMSIPKGNSALRRVM
EETYYHHIYHTVAIEGNTLTLSEIRHILETRYAVP
GKSLEEQNEVIGMHAAMKYINTTLVSRIGSVTISD
VLEIHRRVLGYVDPVEAGRFRTTQVLVGHHIPPHP
QDVEKQMQEFVQWLNSEEAMNLHPVEFAALAHYKL
VYIHPFIDGNGRTSRLLMNLILMQAGYPPITIRKE
QRSDYYHVLEAANEGDVRPFIRFIAKCTETTLDTL
LFATTEYSVALPEAQPNHSGFKETLPVKP


Isotype

IgG

Antibody clone number

3F5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of FICD expression in transfected 293T cell line by HYPE monoclonal antibody (M02), clone 3F5.Lane 1: FICD transfected lysate (Predicted MW: 51.8 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged FICD is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol