686802

antibody from BioLegend

Targeting: NPM1 B23, NPM

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

686802

Provider

BioLegend

Proper citation

BioLegend Cat#686802, RRID:AB_2616887

Product name

Purified anti-Nucleophosmin

Antibody type

Monoclonal

Antigen

Purified recombinant human nucleophosmin (2-265 a.a.) expressed in E. coli.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

P87H10B9

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysate (15 µg protein) from HeLa, Jurkat, and NIH3T3 cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-Nucleophosmin (clone P87H10B9) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.

Conjugate

Unconjugated