686803

antibody from BioLegend

Targeting: NPM1 B23, NPM

Immunocytochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

686803

Provider

BioLegend

Proper citation

BioLegend Cat#686803, RRID:AB_2617022

Product name

Alexa Fluor® 594 anti-Nucleophosmin

Antibody type

Monoclonal

Antigen

Purified recombinant human nucleophosmin (2-265 a.a.) expressed in E. coli.

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

P87H10B9

Vial size

25 µg

Concentration

0.5 μ/ml

Storage

2°C-8°C

Handling

Ambient RT

Other assay

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. The cells were intracellularly stained with 1 µg/ml Alexa Fluor® 594 anti-Nucleophosmin antibody (clone P87H10B9) overnight at 4°C. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.

Conjugate

Yellow dye