MAB1082

antibody from Abnova Corporation

Targeting: NPM1 B23, NPM

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB1082

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB1082, RRID:AB_1578135

Product name

NPM1 monoclonal antibody, clone 5E3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against partial recombinant NPM1.

Isotype

IgG

Antibody clone number

5E3

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Nucleophosmin is required for DNA integrity and p19Arf protein stability.

Colombo E, Bonetti P, Lazzerini Denchi E, Martinelli P, Zamponi R, Marine JC, Helin K, Falini B, Pelicci PG
Molecular and cellular biology 2005 Oct;25(20):8874-86

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Nucleophosmin sets a threshold for p53 response to UV radiation.

Maiguel DA, Jones L, Chakravarty D, Yang C, Carrier F
Molecular and cellular biology 2004 May;24(9):3703-11

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of HeLa and HepG2 cell lysates (5 ug) were resolved by SDS - PAGE , transferred to PVDF membrane and probed with NPM1 monoclonal antibody , clone 5E3 (1 : 1000) (Cat # MAB1082). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemistry of human palatine tonsil tissue by paraffin - embedded. Sample stained with NPM1 monoclonal antibody , clone 5E3 (Cat # MAB1082) at 1 : 100 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)