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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- NB100-56181 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56181, RRID:AB_837812
- Product name
- Rabbit Polyclonal CARD8 Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified. The gene encoding TUCAN produces, by alternative splicing, multiple transcripts, with introns, putatively encoding numerous different protein isoforms. Thus the molecular weight observed by SDS-PAGE can vary depending on the TUCAN form expressed.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Loss-of-function CARD8 mutation causes NLRP3 inflammasome activation and Crohn's disease.
Mao L, Kitani A, Similuk M, Oler AJ, Albenberg L, Kelsen J, Aktay A, Quezado M, Yao M, Montgomery-Recht K, Fuss IJ, Strober W
The Journal of clinical investigation 2018 May 1;128(5):1793-1806
The Journal of clinical investigation 2018 May 1;128(5):1793-1806
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CARD8 Antibody [NB100-56181] - Analysis of CARD8 in tumor cell lines using this antibody. Cell lysates were normalized for total protein content.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CARD8 Antibody [NB100-56181] - Formalin-fixed, paraffin-embedded tissue human colon microarray stained for TUCAN/CARD8 expression at 1:2000 using NB100-56181. A-A2, sucessively higher magnifications of the microarray. A, low magnification overview. A1, differential TUCAN/CARD8 staining between tissue cores is observed. A2, Areas of intense TUCAN/CARD8 staining in a colon carcinoma tissue core. Hematoxylin eosin counterstain.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CARD8 Antibody [NB100-56181] - Formalin-fixed, paraffin-embedded tumor/normal adjacent tissue cores from a human colon tissue microarray stained for TUCAN/CARD8 expression at 1:2000 using NB100-56181. A and B are tumor tissue cores. A1 and B1 are the matched normal adjacent cores from A and B, respectively. Hematoxylin-eosin counterstain.
