Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [3]
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- Product number
- PA5-34087 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- P2Y14 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Percent identity with other species by BLAST analysis: Human (100%) Monkey (95%).
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references P2RY14 cAMP signaling regulates Schwann cell precursor self-renewal, proliferation, and nerve tumor initiation in a mouse model of neurofibromatosis.
Empowering human cardiac progenitor cells by P2Y(14) nucleotide receptor overexpression.
Patritti Cram J, Wu J, Coover RA, Rizvi TA, Chaney KE, Ravindran R, Cancelas JA, Spinner RJ, Ratner N
eLife 2022 Mar 21;11
eLife 2022 Mar 21;11
Empowering human cardiac progenitor cells by P2Y(14) nucleotide receptor overexpression.
Khalafalla FG, Kayani W, Kassab A, Ilves K, Monsanto MM, Alvarez R Jr, Chavarria M, Norman B, Dembitsky WP, Sussman MA
The Journal of physiology 2017 Dec 1;595(23):7135-7148
The Journal of physiology 2017 Dec 1;595(23):7135-7148
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed paraffin-embedded human brain, neurons and glia using a P2RY14/GPR105 polyclonal antibody (Product # PA5-34087). Heat-induced antigen retrieval was performed prior to staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1. P2RY14 is expressed in human neurofibromas and promotes Schwann cell precursor (SCP) self-renewal in vitro. ( A ) Microarray heatmap shows P2ry14 receptor expression in p75 + /EGFR + SCP-like tumor-initiating cells derived from human plexiform neurofibroma tumor cells compared to p75 + /EGFR - SCP-like cells. ( B ) Western blot of human Schwann cells and neurofibroma SCP shows the latter has a 1.9-fold increase in P2ry14 protein expression. ( C ) Immunohistochemistry of human neurofibroma shows P2ry14 expression (DAB staining: brown [P2ry14 positive cells] blue [cell nuclei]). ( D ) Representative fluorescence-activated cells sorting (FACS) plot shows live sorted human plexiform neurofibroma tumor cells. ( E ) Representative FACS plot shows human plexiform neurofibroma tumor cells sorted into p75 + /EGFR + SCP-like tumor-initiating cells (pink square). ( F ) Representative FACS plot shows p75 + /EGFR + SCP-like tumor-initiating cells further sorted into p75 + /EGFR + / P2ry14 - (left, purple square) and P75 + /EGFR + / P2ry14 + (right, blue square). ( G ) Photomicrographs of human neurofibromas dissociated using FACS to yield: unsorted, p75 + /EGFR + / P2ry14 - and P75 + /EGFR + / P2ry14 + cells. ( H ) Quantification of unsorted, p75 + /EGFR + / P2ry14 - and P75 + /EGFR + / P2ry14 + cells plated in sphere medium. (n = 3; two-way ANOVA; primary: **p = 0.0057, ****p < 0.0001; secondary: *p = 0.0487; **p < 0.0024, ****p < 0.0001; tertiary: *p = 0.0321, ***p = 0.0006).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3. P2RY14 is expressed in Schwann cell precursors (SCPs) and Schwann cells (SCs) in vivo in a mouse model of neurofibromatosis. ( A ) Schematic of generation of neurofibroma mice breeding of P2ry14 -/- mice with Nf fl/fl mice to obtain P2ry14 +/- ; Nf1 fl/fl ;Dhh Cre and P2ry14 -/- ; Nf1 fl/fl ;Dhh Cre littermates after several crosses. ( B ) Genotyping confirmation of wild-type (WT) and P2ry14 knockout (KO) alleles. ( C ) Western blot of sciatic nerve and neurofibroma tumors of Nf1 fl/fl ;Dhh Cre and P2ry14 -/- ;Nf1 fl/fl ;Dhh Cre mice show decrease in P2ry14 expression upon P2ry14 knockdown (1- to 0.1-fold decrease in sciatic nerve and 1- to 0.2-fold decrease in neurofibroma tissue). ( D ) Spinal cord (SC) immunofluorescent staining of mouse embryos at embryonic day 12.5 (E12.5) shows P2ry14 expression (beta-galactosidase) co-localization with SOX-10 SCs at the dorsal root ganglion (DRG) and ventral roots (VR). ( E ) Spinal cord (SC) immunofluorescent staining of mouse embryos at E12.5 shows P2ry14 expression (beta-galactosidase) co-localization with SOX-10 SCs at DRG. E1 and E2 insets show enlarged sections of the DRG. ( F ) Immunofluorescent staining of 7-month-old mouse sciatic nerve shows beta-galactosidase positive staining as a confirmation of P2ry14 knock-in; co-labeling of beta-galactosidase and CNPase shows that P2ry14 co-localizes with SCs (inset). ( G ) DAB staining of 7-month-old WT nerve and Nf1 fl/fl ;Dhh Cre mouse neurofibromas (DAB staining: brown [ P