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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [3]
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- Product number
- NB300-574 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-574, RRID:AB_10000607
- Product name
- Mouse Monoclonal Spectrin beta 1 Antibody
- Antibody type
- Monoclonal
- Description
- Unpurified. Detects spectrin from erythrocytes, brain and muscle cells. This has been shown to specifically detect the two known alternatively spliced forms of spectrin, beta-1 epsilon-1, present in erythrocytes, and beta-1 epsilon-2, present in nerve and striated muscle cells. It does not cross-react with alpha-2 spectrin or either of the fodrin subunits.
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100uL
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references A comparative study of N-glycolylneuraminic acid (Neu5Gc) and cytotoxic T cell (CT) carbohydrate expression in normal and dystrophin-deficient dog and human skeletal muscle.
Martin PT, Golden B, Okerblom J, Camboni M, Chandrasekharan K, Xu R, Varki A, Flanigan KM, Kornegay JN
PloS one 2014;9(2):e88226
PloS one 2014;9(2):e88226
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of 25 ug of Hela cell lysates.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis showing positive staining in the cytoplasm and membrane of Mouse skeletal muscle (right) compared with a negative control in the absence of primary antibody (left).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis showing positive staining in the cytoplasm of Human prostate carcinoma (right) compared with a negative control in the absence of primary antibody (left).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis showing positive staining in the membrane of Human skeletal muscle (right) compared with a negative control in the absence of primary antibody (left).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of SH-SY5Y cells compared to an isotype control (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of NIH/3T3 cells compared to an isotype control (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of Hela cells compared to an isotype control (blue).
