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LearnM01930-1
antibody from Boster Biological Technology
Targeting: SMC3
BAM, bamacan, CSPG6, HCAP, SMC3L1
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- M01930-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-SMC3 Antibody Picoband™ (monoclonal, 4C12)
- Antibody type
- Monoclonal
- Description
- Mouse IgG monoclonal antibody for SMC3 detection. Tested with WB, IHC-P in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4C12
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20℃ for one year. After reconstitution, at 4℃ for one month. It can also be aliquoted and stored frozen at -20℃ for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of SMC3 using anti-SMC3 antibody (M01930-1). SMC3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-SMC3 Antibody (M01930-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
- Additional image
