Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- LS-C782167 - Provider product page
- Provider
- LSBio
- Product name
- SMC3 / HCAP Antibody (clone 4C12) LS-C782167
- Antibody type
- Monoclonal
- Description
- Protein G affinity chromatography
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4C12
- Storage
- After reconstitution, store at 4°C for up to 1 month. Long-term: aliquot and store at -20°C. Avoid freeze-thaws cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) A549, 3) MCF7 and 4) MDA-MB-453 lysate with SMC3 antibody at 0.5ug/ml. Predicted molecular weight ~141 kDa.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC testing of FFPE human intestinal cancer with SMC3 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC testing of FFPE human lung cancer with SMC3 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.