Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-50581 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Nicotinamide N-Methyltransferase/NNMT Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) [NBP2-50581] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NNMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: A549 cell lysate
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) [NBP2-50581] - Flow cytometry analysis of NNMT in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
