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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- TA502538 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502538, RRID:AB_11126363
- Product name
- NNMT mouse monoclonal antibody, clone OTI2A3 (formerly 2A3)
- Antibody type
- Monoclonal
- Description
- NNMT mouse monoclonal antibody, clone OTI2A3 (formerly 2A3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- NNMT
- Isotype
- IgG
- Antibody clone number
- OTI2A3
- Vial size
- 100 µl
- Concentration
- 0.16 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NNMT (RC200641, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NNMT.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NNMT monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-NNMT mouse monoclonal antibody (TA502538) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NNMT(RC200641).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502538)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC200641 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NNMT antibody(TA502538), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-NNMT antibody(TA502538),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
