AF1085

antibody from Novus Biologicals

Targeting: ARTN ENOVIN, EVN, NBN

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1085

Provider

Novus Biologicals

Product name

Goat Polyclonal Artemin Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse Artemin in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human GDNF and recombinant rat GDNF is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

GDNF, Neurturin, and Artemin Activate and Sensitize Bone Afferent Neurons and Contribute to Inflammatory Bone Pain.

Nencini S, Ringuet M, Kim DH, Greenhill C, Ivanusic JJ
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 May 23;38(21):4899-4911

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Tumor-Induced Generation of Splenic Erythroblast-like Ter-Cells Promotes Tumor Progression.

Han Y, Liu Q, Hou J, Gu Y, Zhang Y, Chen Z, Fan J, Zhou W, Qiu S, Zhang Y, Dong T, Li N, Jiang Z, Zhu H, Zhang Q, Ma Y, Zhang L, Wang Q, Yu Y, Li N, Cao X
Cell 2018 Apr 19;173(3):634-648.e12

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The aryl hydrocarbon receptor AhR links atopic dermatitis and air pollution via induction of the neurotrophic factor artemin.

Hidaka T, Ogawa E, Kobayashi EH, Suzuki T, Funayama R, Nagashima T, Fujimura T, Aiba S, Nakayama K, Okuyama R, Yamamoto M
Nature immunology 2017 Jan;18(1):64-73

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Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence.

Ibiza S, García-Cassani B, Ribeiro H, Carvalho T, Almeida L, Marques R, Misic AM, Bartow-McKenney C, Larson DM, Pavan WJ, Eberl G, Grice EA, Veiga-Fernandes H
Nature 2016 Jul 21;535(7612):440-443

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Autocrine regulation of early embryonic development by the artemin-GFRA3 (GDNF family receptor-alpha 3) signaling system in mice.

Li J, Klein C, Liang C, Rauch R, Kawamura K, Hsueh AJ
FEBS letters 2009 Aug 6;583(15):2479-85

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Artemin overexpression in skin enhances expression of TRPV1 and TRPA1 in cutaneous sensory neurons and leads to behavioral sensitivity to heat and cold.

Elitt CM, McIlwrath SL, Lawson JJ, Malin SA, Molliver DC, Cornuet PK, Koerber HR, Davis BM, Albers KM
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Aug 16;26(33):8578-87

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by Artemin and Neutralization by Mouse Artemin Antibody. Recombinant Mouse Artemin (Catalog # 1085-AR) stimulates proliferation in the SH-SY5Y human neuroblastoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Artemin (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Artemin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1085). The ND₅₀ is typically 0.1-0.3 µg/mL.