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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AM33447PU-N - Provider product page
- Provider
- OriGene
- Product name
- Artemin (ARTN) mouse monoclonal antibody, clone 2A5, Aff - Purified
- Antibody type
- Monoclonal
- Description
- Artemin (ARTN) mouse monoclonal antibody, clone 2A5, Aff - Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ARTN
- Isotype
- IgG
- Antibody clone number
- 2A5
- Vial size
- 100 µg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot testing of purified recombinant Artemin by anti-Artemin antibody 2A5. For analysis HRP-conjugated 2A5 antibody was used. 200 ng of purified recombinant Artemin expressed by CHOEBNALT85 cell line was loaded per lane. Lane 1: Reduced conditions; Line 2: Non-reduced conditions; Lane 3: Protein marker (8003, Naxo)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of GFRA3-Ret51 receptor complex bound Artemin. ? Human U2OS cells were transfected with GFRa3-Ret51 expression vector followed by treatment with 100 nM Artemin. Artemin bound to the GFRa3-Ret51 receptor complex was detected by anti-Artemin antibody clone 2A5. Goat anti-mouse Dylight 488 conjugated antibody (dilution 1:1000) was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for Artemin and blue for nuclei. A. Artemin bound to the GFRa3-Ret51 receptor complex; B. Negative control - GFRa3-Ret51 receptor expressing U2OS cells
- Validation comment
- IF
