GTX12169

antibody from GeneTex

Targeting: HDAC2 KDAC2, RPD3, YAF1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

GTX12169

Provider

GeneTex

Proper citation

GeneTex Cat#GTX12169, RRID:AB_381379

Product name

HDAC2 antibody [HDAC2-62]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian

Host

Mouse

References

Submitted references

LncRNA ANCR down-regulation promotes TGF-β-induced EMT and metastasis in breast cancer.

Li Z, Dong M, Fan D, Hou P, Li H, Liu L, Lin C, Liu J, Su L, Wu L, Li X, Huang B, Lu J, Zhang Y
Oncotarget 2017 Sep 15;8(40):67329-67343

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HDAC2 and TXNL1 distinguish aneuploid from diploid colorectal cancers.

Gemoll T, Roblick UJ, Szymczak S, Braunschweig T, Becker S, Igl BW, Bruch HP, Ziegler A, Hellman U, Difilippantonio MJ, Ried T, Jörnvall H, Auer G, Habermann JK
Cellular and molecular life sciences : CMLS 2011 Oct;68(19):3261-74

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Cell line lysates were separated on SDS-PAGE and probed with 0.5 μg/mL Monoclonal Anti-Histone Deacetylase 2 Clone: HDAC2-62 (GTX12169). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate Lanes 1. HEK-293T 2. HeLa 3. JURKAT 4. K562 5. Neuro-2a 6. NIH-3T3

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Monoclonal Anti-HDAC2 Clone: HDAC2-62 (GTX12169) was used to immunoprecipitate HDAC2 from HeLa whole cells extract. Detection antibody: Rabbit Anti-HDAC2. Lanes 1. 5 μg antibody 2. 2.5 μg antibody 3. Negative control: 5 μg/mL non-relevant antibody

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone Fixed cells were stained with 10 μg/mL Monoclonal Anti-Histone Deacetylase 2 Clone: HDAC2-62 (GTX12169). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjμgate.