680104

antibody from BioLegend

Targeting: HDAC2 KDAC2, RPD3, YAF1

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

680104

Provider

BioLegend

Proper citation

BioLegend Cat#680104, RRID:AB_2632842

Product name

Go-ChIP-Grade™ Purified anti-HDAC2

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

13G8C67

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Other assay

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay plate by loading 3 µg of cross-linked chromatin samples from Jurkat cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-HDAC2 Antibody (clone 13G8C67), B) equal amount of Purified Mouse IgG1, κ Isotype Control Antibody (clone MG1-45), or C) competitor's ChIP-grade Purified anti-HDAC2 Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human FOSL1 (Fos like 1) gene region, which is known to be bound to HDAC2. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.

Conjugate

Unconjugated