H00003066-D01P

antibody from Abnova Corporation

Targeting: HDAC2 KDAC2, RPD3, YAF1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003066-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003066-D01P, RRID:AB_1674140

Product name

HDAC2 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human HDAC2 protein.

Antigen sequence

MRSPPCGLLRWFGGPLLASWCRCHLRFRAFGTSAG
WYRAFPAPPPLLPPACPSPRDYRPHVSLSPFLSRP
SRGGSSSSSSSRRRSPVAAVAGEPMAYSQGGGKKK
VCYYYDGDIGNYYYGQGHPMKPHRIRMTHNLLLNY
GLYRKMEIYRPHKATAEEMTKYHSDEYIKFLRSIR
PDNMSEYSKQMQRFNVGEDCPVFDGLFEFCQLSTG
GSVAGAVKLNRQQTDMAVNWAGGLHHAKKSEASGF
CYVNDIVLAILELLKYHQRVLYIDIDIHHGDGVEE
AFYTTDRVMTVSFHKYGEYFPGTGDLRDIGAGKGK
YYAVNFPMRDGIDDESYGQIFKPIISKVMEMYQPS
AVVLQCGADSLSGDRLGCFNLTVKGHAKCVEVVKT
FNLPLLMLGGGGYTIRNVARCWTYETAVALDCEIP
NELPYNDYFEYFGPDFKLHISPSNMTNQNTPEYME
KIKQRLFENLRMLPHAPGVQMQAIPEDAVHEDSGD
EDGEDPDKRISIRASDKRIACDEEFSDSEDEGEGG
RRNVADHKKGAKKARIEEDKKETEDKKTDVKEEDK
SKDNSGEKTDTKGTKSEQLSNP


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of HDAC2 expression in transfected 293T cell line (H00003066-T02) by HDAC2 MaxPab polyclonal antibody.Lane 1: HDAC2 transfected lysate(64.02 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to HDAC2 on HeLa cell. [antibody concentration 30 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between HDAC2 and PPARD. HeLa cells were stained with anti-HDAC2 rabbit purified polyclonal 1:1200 and anti-PPARD mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)