MAB7679

antibody from R&D Systems

Targeting: HDAC2 KDAC2, RPD3, YAF1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB7679

Provider

R&D Systems

Product name

Human/Rat Histone Deacetylase 2/HDAC2 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Histone Deacetylase 2/HDAC-2 in direct ELISAs and human and rat Histone Deacetylase 2/HDAC-2 Western blots. In direct ELISAs, no cross-reactivity with recombinant human HDAC1 is observed.

Reactivity

Human, Rat

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Q92769


Isotype

IgG

Antibody clone number

806116

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human and Rat Histone Deacetylase 2/HDAC2 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, SH-SY5Y human neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Histone Deacetylase 2/HDAC2 Monoclonal Antibody (Catalog # MAB7679) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Histone Deacetylase 2/HDAC2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Histone Deacetylase 2/HDAC2 by Simple Western^TM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.5 mg/mL. A specific band was detected for Histone Deacetylase 2/HDAC2 at approximately 98 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human/Rat Histone Deacetylase 2/HDAC2 Monoclonal Antibody (Catalog # MAB7679). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.