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- Antigen structure
- References [28]
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- Western blot [3]
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- Product number
- NB100-177 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-177, RRID:AB_10001856
- Product name
- Mouse Monoclonal Rad51C Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Does not cross-react with Rad51B, Rad51D, Rad51, XRCC2, or XRCC3 in Western analysis.
- Reactivity
- Human, Mouse, Simian, Yeast
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The CHK1 Inhibitor Prexasertib Exhibits Monotherapy Activity in High-Grade Serous Ovarian Cancer Models and Sensitizes to PARP Inhibition.
Human RAD51 paralogue RAD51C fosters repair of alkylated DNA by interacting with the ALKBH3 demethylase.
Characterisation of the novel deleterious RAD51C p.Arg312Trp variant and prioritisation criteria for functional analysis of RAD51C missense changes.
Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells.
BRCA2 antagonizes classical and alternative nonhomologous end-joining to prevent gross genomic instability.
MUS81 nuclease activity is essential for replication stress tolerance and chromosome segregation in BRCA2-deficient cells.
Breast cancer-associated missense mutants of the PALB2 WD40 domain, which directly binds RAD51C, RAD51 and BRCA2, disrupt DNA repair.
RAD51C-deficient cancer cells are highly sensitive to the PARP inhibitor olaparib.
Rad51 paralog complexes BCDX2 and CX3 act at different stages in the BRCA1-BRCA2-dependent homologous recombination pathway.
Systematic screen identifies miRNAs that target RAD51 and RAD51D to enhance chemosensitivity.
BRCA2 is epistatic to the RAD51 paralogs in response to DNA damage.
Sirtuin 6 (SIRT6) rescues the decline of homologous recombination repair during replicative senescence.
MiR-96 downregulates REV1 and RAD51 to promote cellular sensitivity to cisplatin and PARP inhibition.
Discovery of a novel function for human Rad51: maintenance of the mitochondrial genome.
Cellular redistribution of Rad51 in response to DNA damage: novel role for Rad51C.
Dysfunctional homologous recombination mediates genomic instability and progression in myeloma.
Chronic hypoxia decreases synthesis of homologous recombination proteins to offset chemoresistance and radioresistance.
RAD51C deficiency in mice results in early prophase I arrest in males and sister chromatid separation at metaphase II in females.
Differential regulation of short- and long-tract gene conversion between sister chromatids by Rad51C.
Cellular localization of human Rad51C and regulation of ubiquitin-mediated proteolysis of Rad51.
RAD51C is required for Holliday junction processing in mammalian cells.
Defective DNA strand break repair after DNA damage in prostate cancer cells: implications for genetic instability and prostate cancer progression.
Role of mammalian RAD51L2 (RAD51C) in recombination and genetic stability.
Inactivation of the RAD51 recombination pathway stimulates UV-induced mutagenesis in mammalian cells.
Autoantibodies in sera of pancreatic cancer patients identify recombination factor Rad51 as a tumour-associated antigen.
Complex formation by the human RAD51C and XRCC3 recombination repair proteins.
Isolation and characterization of RAD51C, a new human member of the RAD51 family of related genes.
Isolation and characterization of RAD51C, a new human member of the RAD51 family of related genes.
Parmar K, Kochupurakkal BS, Lazaro JB, Wang ZC, Palakurthi S, Kirschmeier PT, Yang C, Sambel LA, Färkkilä A, Reznichenko E, Reavis HD, Dunn CE, Zou L, Do KT, Konstantinopoulos PA, Matulonis UA, Liu JF, D'Andrea AD, Shapiro GI
Clinical cancer research : an official journal of the American Association for Cancer Research 2019 Oct 15;25(20):6127-6140
Clinical cancer research : an official journal of the American Association for Cancer Research 2019 Oct 15;25(20):6127-6140
Human RAD51 paralogue RAD51C fosters repair of alkylated DNA by interacting with the ALKBH3 demethylase.
Mohan M, Akula D, Dhillon A, Goyal A, Anindya R
Nucleic acids research 2019 Dec 16;47(22):11729-11745
Nucleic acids research 2019 Dec 16;47(22):11729-11745
Characterisation of the novel deleterious RAD51C p.Arg312Trp variant and prioritisation criteria for functional analysis of RAD51C missense changes.
Gayarre J, Martín-Gimeno P, Osorio A, Paumard B, Barroso A, Fernández V, de la Hoya M, Rojo A, Caldés T, Palacios J, Urioste M, Benítez J, García MJ
British journal of cancer 2017 Sep 26;117(7):1048-1062
British journal of cancer 2017 Sep 26;117(7):1048-1062
Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells.
Wu D, Huang CJ, Jiao XF, Ding ZM, Zhang JY, Chen F, Wang YS, Li X, Huo LJ
Oncotarget 2017 Oct 24;8(51):88630-88644
Oncotarget 2017 Oct 24;8(51):88630-88644
BRCA2 antagonizes classical and alternative nonhomologous end-joining to prevent gross genomic instability.
Han J, Ruan C, Huen MSY, Wang J, Xie A, Fu C, Liu T, Huang J
Nature communications 2017 Nov 13;8(1):1470
Nature communications 2017 Nov 13;8(1):1470
MUS81 nuclease activity is essential for replication stress tolerance and chromosome segregation in BRCA2-deficient cells.
Lai X, Broderick R, Bergoglio V, Zimmer J, Badie S, Niedzwiedz W, Hoffmann JS, Tarsounas M
Nature communications 2017 Jul 17;8:15983
Nature communications 2017 Jul 17;8:15983
Breast cancer-associated missense mutants of the PALB2 WD40 domain, which directly binds RAD51C, RAD51 and BRCA2, disrupt DNA repair.
Park JY, Singh TR, Nassar N, Zhang F, Freund M, Hanenberg H, Meetei AR, Andreassen PR
Oncogene 2014 Oct 2;33(40):4803-12
Oncogene 2014 Oct 2;33(40):4803-12
RAD51C-deficient cancer cells are highly sensitive to the PARP inhibitor olaparib.
Min A, Im SA, Yoon YK, Song SH, Nam HJ, Hur HS, Kim HP, Lee KH, Han SW, Oh DY, Kim TY, O'Connor MJ, Kim WH, Bang YJ
Molecular cancer therapeutics 2013 Jun;12(6):865-77
Molecular cancer therapeutics 2013 Jun;12(6):865-77
Rad51 paralog complexes BCDX2 and CX3 act at different stages in the BRCA1-BRCA2-dependent homologous recombination pathway.
Chun J, Buechelmaier ES, Powell SN
Molecular and cellular biology 2013 Jan;33(2):387-95
Molecular and cellular biology 2013 Jan;33(2):387-95
Systematic screen identifies miRNAs that target RAD51 and RAD51D to enhance chemosensitivity.
Huang JW, Wang Y, Dhillon KK, Calses P, Villegas E, Mitchell PS, Tewari M, Kemp CJ, Taniguchi T
Molecular cancer research : MCR 2013 Dec;11(12):1564-73
Molecular cancer research : MCR 2013 Dec;11(12):1564-73
BRCA2 is epistatic to the RAD51 paralogs in response to DNA damage.
Jensen RB, Ozes A, Kim T, Estep A, Kowalczykowski SC
DNA repair 2013 Apr 1;12(4):306-11
DNA repair 2013 Apr 1;12(4):306-11
Sirtuin 6 (SIRT6) rescues the decline of homologous recombination repair during replicative senescence.
Mao Z, Tian X, Van Meter M, Ke Z, Gorbunova V, Seluanov A
Proceedings of the National Academy of Sciences of the United States of America 2012 Jul 17;109(29):11800-5
Proceedings of the National Academy of Sciences of the United States of America 2012 Jul 17;109(29):11800-5
MiR-96 downregulates REV1 and RAD51 to promote cellular sensitivity to cisplatin and PARP inhibition.
Wang Y, Huang JW, Calses P, Kemp CJ, Taniguchi T
Cancer research 2012 Aug 15;72(16):4037-46
Cancer research 2012 Aug 15;72(16):4037-46
Discovery of a novel function for human Rad51: maintenance of the mitochondrial genome.
Sage JM, Gildemeister OS, Knight KL
The Journal of biological chemistry 2010 Jun 18;285(25):18984-90
The Journal of biological chemistry 2010 Jun 18;285(25):18984-90
Cellular redistribution of Rad51 in response to DNA damage: novel role for Rad51C.
Gildemeister OS, Sage JM, Knight KL
The Journal of biological chemistry 2009 Nov 13;284(46):31945-52
The Journal of biological chemistry 2009 Nov 13;284(46):31945-52
Dysfunctional homologous recombination mediates genomic instability and progression in myeloma.
Shammas MA, Shmookler Reis RJ, Koley H, Batchu RB, Li C, Munshi NC
Blood 2009 Mar 5;113(10):2290-7
Blood 2009 Mar 5;113(10):2290-7
Chronic hypoxia decreases synthesis of homologous recombination proteins to offset chemoresistance and radioresistance.
Chan N, Koritzinsky M, Zhao H, Bindra R, Glazer PM, Powell S, Belmaaza A, Wouters B, Bristow RG
Cancer research 2008 Jan 15;68(2):605-14
Cancer research 2008 Jan 15;68(2):605-14
RAD51C deficiency in mice results in early prophase I arrest in males and sister chromatid separation at metaphase II in females.
Kuznetsov S, Pellegrini M, Shuda K, Fernandez-Capetillo O, Liu Y, Martin BK, Burkett S, Southon E, Pati D, Tessarollo L, West SC, Donovan PJ, Nussenzweig A, Sharan SK
The Journal of cell biology 2007 Feb 26;176(5):581-92
The Journal of cell biology 2007 Feb 26;176(5):581-92
Differential regulation of short- and long-tract gene conversion between sister chromatids by Rad51C.
Nagaraju G, Odate S, Xie A, Scully R
Molecular and cellular biology 2006 Nov;26(21):8075-86
Molecular and cellular biology 2006 Nov;26(21):8075-86
Cellular localization of human Rad51C and regulation of ubiquitin-mediated proteolysis of Rad51.
Bennett BT, Knight KL
Journal of cellular biochemistry 2005 Dec 15;96(6):1095-109
Journal of cellular biochemistry 2005 Dec 15;96(6):1095-109
RAD51C is required for Holliday junction processing in mammalian cells.
Liu Y, Masson JY, Shah R, O'Regan P, West SC
Science (New York, N.Y.) 2004 Jan 9;303(5655):243-6
Science (New York, N.Y.) 2004 Jan 9;303(5655):243-6
Defective DNA strand break repair after DNA damage in prostate cancer cells: implications for genetic instability and prostate cancer progression.
Fan R, Kumaravel TS, Jalali F, Marrano P, Squire JA, Bristow RG
Cancer research 2004 Dec 1;64(23):8526-33
Cancer research 2004 Dec 1;64(23):8526-33
Role of mammalian RAD51L2 (RAD51C) in recombination and genetic stability.
French CA, Masson JY, Griffin CS, O'Regan P, West SC, Thacker J
The Journal of biological chemistry 2002 May 31;277(22):19322-30
The Journal of biological chemistry 2002 May 31;277(22):19322-30
Inactivation of the RAD51 recombination pathway stimulates UV-induced mutagenesis in mammalian cells.
Lambert S, Lopez BS
Oncogene 2002 Jun 6;21(25):4065-9
Oncogene 2002 Jun 6;21(25):4065-9
Autoantibodies in sera of pancreatic cancer patients identify recombination factor Rad51 as a tumour-associated antigen.
Maacke H, Hundertmark C, Miska S, Voss M, Kalthoff H, Stürzbecher HW
Journal of cancer research and clinical oncology 2002 Apr;128(4):219-22
Journal of cancer research and clinical oncology 2002 Apr;128(4):219-22
Complex formation by the human RAD51C and XRCC3 recombination repair proteins.
Masson JY, Stasiak AZ, Stasiak A, Benson FE, West SC
Proceedings of the National Academy of Sciences of the United States of America 2001 Jul 17;98(15):8440-6
Proceedings of the National Academy of Sciences of the United States of America 2001 Jul 17;98(15):8440-6
Isolation and characterization of RAD51C, a new human member of the RAD51 family of related genes.
Dosanjh MK, Collins DW, Fan W, Lennon GG, Albala JS, Shen Z, Schild D
Nucleic acids research 1998 Mar 1;26(5):1179-84
Nucleic acids research 1998 Mar 1;26(5):1179-84
Isolation and characterization of RAD51C, a new human member of the RAD51 family of related genes.
Dosanjh MK, Collins DW, Fan W, Lennon GG, Albala JS, Shen Z, Schild D
Nucleic acids research 1998 Mar 1;26(5):1179-84
Nucleic acids research 1998 Mar 1;26(5):1179-84
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Rad51C Antibody (2H11/6) [NB100-177] - Lane view shows a specific band for Rad51C in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rad51C Antibody (2H11/6) [NB100-177] - Rad51C detected in HEK293 lysate using NB 100-177. Photo courtesy of B.T. Bennett & K. Knight, University of Massachusetts Medical School.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rad51C Antibody (2H11/6) [NB100-177] - Analysis of RAD51c in 1) Hela WCE 2) HepG2 WCE 3) Cos-7 WCE 4) Hek293 WCE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Rad51C Antibody (2H11/6) [NB100-177] - Intracellular flow cytometric staining of 1 x 10^6 HeLa cells using Rad51C antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
