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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- TA800541 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA800541, RRID:AB_2625521
- Product name
- PRR11 mouse monoclonal antibody, clone OTI3C8 (formerly 3C8)
- Antibody type
- Monoclonal
- Description
- PRR11 mouse monoclonal antibody, clone OTI3C8 (formerly 3C8)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PRR11
- Isotype
- IgG
- Antibody clone number
- OTI3C8
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
Submitted references Global Membrane Protein Interactome Analysis using In vivo Crosslinking and Mass Spectrometry-based Protein Correlation Profiling.
Larance M, Kirkwood KJ, Tinti M, Brenes Murillo A, Ferguson MA, Lamond AI
Molecular & cellular proteomics : MCP 2016 Jul;15(7):2476-90
Molecular & cellular proteomics : MCP 2016 Jul;15(7):2476-90
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRR11 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRR11 (RC201889, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRR11.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-PRR11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800541)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PRR11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800541)
- Validation comment
- IHC
