Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-113175 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRR11 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 5 mg/mL
- Storage
- -20°C or -80°C if preferred
Submitted references PRR11 promotes cell proliferation by regulating PTTG1 through interacting with E2F1 transcription factor in pan-cancer.
Zhang H, He Z, Qiu L, Wei J, Gong X, Xian M, Chen Z, Cui Y, Fu S, Zhang Z, Hu B, Zhang X, Lin S, Du H
Frontiers in molecular biosciences 2022;9:877320
Frontiers in molecular biosciences 2022;9:877320
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PRR11 using a polyclonal antibody (Product # PA5-113175) at a dilution of 2 µg/mL. A goat anti-rabbit polyclonal secondary antibody at a dilution of 1:50,000 was used for detection in mouse heart tissue. Observed band: 41 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PRR11 using a polyclonal antibody (Product # PA5-113175) at a dilution of 2 µg/mL. A goat anti-rabbit polyclonal secondary antibody at a dilution of 1:50,000 was used for detection in mouse heart tissue. Observed band: 41 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemichal analysis of PRR11 in PC-3 cells using a Polyclonal antibody (Product # PA5-113175) at a dilution of 1:100. An Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibdoy was used.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of paraffin-embeded PRR11 in human pancreatic cancer using a PRR11 Polyclonal antibody (Product # PA5-113175) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of paraffin-embeded PRR11 in human lung cancer tissue using a PRR11 polyclonal antibody (Product # PA5-113175) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 6 PRR11 promotes the expression level of PTTG1 by interacting with E2F1. (A) Localization for PRR11 gene was analyzed using GeneCards ( https://www.genecards.org/ ). (B) Immunofluorescence localization of PRR11. (C) Expression levels of PTTG1 after interfering E2F1. (D) ChIP-qPCR of the PTTG1 promoter region. (E) Expression levels of E2F1 and PRR11 in NCI-H460 cells transfected with siNC or siPRR11 were examined by Western blotting. (F) Co-IP of PRR11 and E2F1 in NCI-H460 cells. N = 3. * p < 0.05, ** p < 0.01, and *** p < 0.001.