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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- ADI-SPA-901-D - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ADI-SPA-901-D, RRID:AB_2039202
- Product name
- HSF1 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full length protein
- Description
- Protein A affinity purified.
- Reactivity
- Human, Mouse, Rat, Rabbit, Simian
- Host
- Rabbit
- Vial size
- 50 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references Thiopental protects human neuroblastoma cells from apoptotic cell death - Potential role of heat shock protein 70.
Disruption of polyubiquitin gene Ubc leads to attenuated resistance against arsenite-induced toxicity in mouse embryonic fibroblasts.
Expression of heat shock transcription factor 1 and its downstream target protein T-cell death associated gene 51 in the spinal cord of a mouse model of amyotrophic lateral sclerosis.
Chemical and biological approaches synergize to ameliorate protein-folding diseases.
TNFalpha mediates susceptibility to heat-induced apoptosis by protein phosphatase-mediated inhibition of the HSF1/hsp70 stress response.
Postinsult treatment with lithium reduces brain damage and facilitates neurological recovery in a rat ischemia/reperfusion model.
17-beta-estradiol induces heat shock proteins in brain arteries and potentiates ischemic heat shock protein induction in glia and neurons.
Role of cyclopentenone prostaglandins in rat carrageenin pleurisy.
Evidence for a mechanism of repression of heat shock factor 1 transcriptional activity by a multichaperone complex.
Signal transducer and activator of transcription-1 and heat shock factor-1 interact and activate the transcription of the Hsp-70 and Hsp-90beta gene promoters.
Roesslein M, Froehlich C, Miltenberger V, Ulbrich F, Goebel U, Loop T
Life sciences 2015 Oct 15;139:40-5
Life sciences 2015 Oct 15;139:40-5
Disruption of polyubiquitin gene Ubc leads to attenuated resistance against arsenite-induced toxicity in mouse embryonic fibroblasts.
Kim MN, Choi J, Ryu HW, Ryu KY
Biochimica et biophysica acta 2015 May;1853(5):996-1009
Biochimica et biophysica acta 2015 May;1853(5):996-1009
Expression of heat shock transcription factor 1 and its downstream target protein T-cell death associated gene 51 in the spinal cord of a mouse model of amyotrophic lateral sclerosis.
Mimoto T, Morimoto N, Miyazaki K, Kurata T, Sato K, Ikeda Y, Abe K
Brain research 2012 Dec 7;1488:123-31
Brain research 2012 Dec 7;1488:123-31
Chemical and biological approaches synergize to ameliorate protein-folding diseases.
Mu TW, Ong DS, Wang YJ, Balch WE, Yates JR 3rd, Segatori L, Kelly JW
Cell 2008 Sep 5;134(5):769-81
Cell 2008 Sep 5;134(5):769-81
TNFalpha mediates susceptibility to heat-induced apoptosis by protein phosphatase-mediated inhibition of the HSF1/hsp70 stress response.
Schett G, Steiner CW, Xu Q, Smolen JS, Steiner G
Cell death and differentiation 2003 Oct;10(10):1126-36
Cell death and differentiation 2003 Oct;10(10):1126-36
Postinsult treatment with lithium reduces brain damage and facilitates neurological recovery in a rat ischemia/reperfusion model.
Ren M, Senatorov VV, Chen RW, Chuang DM
Proceedings of the National Academy of Sciences of the United States of America 2003 May 13;100(10):6210-5
Proceedings of the National Academy of Sciences of the United States of America 2003 May 13;100(10):6210-5
17-beta-estradiol induces heat shock proteins in brain arteries and potentiates ischemic heat shock protein induction in glia and neurons.
Lu A, Ran RQ, Clark J, Reilly M, Nee A, Sharp FR
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2002 Feb;22(2):183-95
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2002 Feb;22(2):183-95
Role of cyclopentenone prostaglandins in rat carrageenin pleurisy.
Ianaro A, Ialenti A, Maffia P, Pisano B, Di Rosa M
FEBS letters 2001 Nov 9;508(1):61-6
FEBS letters 2001 Nov 9;508(1):61-6
Evidence for a mechanism of repression of heat shock factor 1 transcriptional activity by a multichaperone complex.
Guo Y, Guettouche T, Fenna M, Boellmann F, Pratt WB, Toft DO, Smith DF, Voellmy R
The Journal of biological chemistry 2001 Dec 7;276(49):45791-9
The Journal of biological chemistry 2001 Dec 7;276(49):45791-9
Signal transducer and activator of transcription-1 and heat shock factor-1 interact and activate the transcription of the Hsp-70 and Hsp-90beta gene promoters.
Stephanou A, Isenberg DA, Nakajima K, Latchman DS
The Journal of biological chemistry 1999 Jan 15;274(3):1723-8
The Journal of biological chemistry 1999 Jan 15;274(3):1723-8
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis of HSF1: Lane 1: Molecular Weight Marker, Lane 2: HSF1 (human), (recombinant) (Prod. No. ADI-SPP-900), Lane 3: HeLa Cell Lysate (Prod. No. ADI-LYC-HL100), Lane 4: HeLa Cell Lysate (Heat Shocked) (Prod. No. ADI-LYC-HL101), Lane 5: 3T3 Cell Lysate (Prod. No. ADI-LYC-3T100), Lane 6: PC-12 Cell Lysate (Prod. No. ADI-LYC-PC100), Lane 7: RK-13 Cell Lysate, Lane 8: Vero Cell Lysate
