CF811748

antibody from Invitrogen Antibodies

Targeting: GPR17

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

CF811748

Provider

Invitrogen Antibodies

Product name

GPR17 Monoclonal Antibody (OTI10B8), TrueMAB™

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

OTI10B8

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of lysates of 293T cells transfected with (1)pCINEO-hGPR17, (2)pcDNA3.1-mGPR17, (3)pcDNA3.1-rGPR17, (4)pcDNA3.1, or (5)PCINEO plasmids using anti-GPR17 Mouse monoclonal antibody. (1:500)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of lysates of 293T cells transfected with (1)pCINEO-hGPR17, (2)pcDNA3.1-mGPR17, (3)pcDNA3.1-rGPR17, (4)pcDNA3.1, or (5)PCINEO plasmids using anti-GPR17 Mouse monoclonal antibody. (1:500)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical staining of paraffin-embedded cell pellets of 293T cells transfected with pcDNA3.1-mGPR17, pcDNA3.1-rGPR17 or pcDNA3.1 plasmids using anti-GPR17 Mouse monoclonal antibody (Heat-induced epitope retrieval by Tris-EDTA buffer (pH8.0) at 120°C for 2.5 min)(1:150).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical staining of paraffin-embedded cell pellets of 293T cells transfected with pCINEO-hGPR17 or pCINEO plasmids using anti-GPR17 Mouse monoclonal antibody (Heat-induced epitope retrieval by Tris-EDTA buffer (pH8.0) at 120°C for 2.5 min)(1:150).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human adult brain tissue within the normal limits using anti-GPR17 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA811748)(1:300)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human embryonic cerebellum within the normal limits using anti-GPR17 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA811748)(1:150)(1:300)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded mouse cerebrum tissue within the normal limits using anti-GPR17 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA811748)(1:200)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded rat cerebrum tissue within the normal limits using anti-GPR17 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA811748)(1:200)