PAB21854

antibody from Abnova Corporation

Targeting: WDR33 FLJ11294, NET14, WDC146

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21854

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21854, RRID:AB_10965218

Product name

WDR33 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant WDR33.

Antigen sequence

AQQQAMQQLTFDGKRMRKAVNRKTIDYNPSVIKYL
ENRIWQRDQRDMRAIQPDAGYYNDLVPPIGMLNNP
MNAVTTKFVRTSTNKVKCPVFVVRWTPEGRRLVTG
ASSGEFTLWNGLTFNF


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with WDR33 polyclonal antibody (Cat # PAB21854).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with WDR33 polyclonal antibody (Cat # PAB21854) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human colon with WDR33 polyclonal antibody (Cat # PAB21854) shows strong nuclear positivity in glandular cells at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)