11744-1-AP

antibody from Invitrogen Antibodies

Targeting: IFT81 CDV-1R, CDV1, MGC4027

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11744-1-AP

Provider

Invitrogen Antibodies

Product name

IFT81 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: VRRLREECL QEESRYHYTN CMIKNLEVQL RRATDEMKAY ISSDQQEKRK AIREQYTKNT AEQENLGKKL REKQKVIRES HGPNMKQAKM WRDLEQLMEC KKQCFLKQQS QTSIGQVIQE GGEDRLIL (550-676 aa encoded by BC029349)

Reactivity

Human, Mouse, Canine

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.33 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IF result (the base and tip of cilia) of anti-IFT81 (11744-1-AP, 1:50) with serum-starved hTERT-RPE1 (PFA fixed) by Dr. Moshe Kim.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IF result (localization to the spindle poles) of anti-IFT81 (11744-1-AP, 1:50) with metAPhase hTERT-RPE1 cells (MeOH fixed) byDr. Moshe Kim.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent images of MDCK cells stained with IFT81 rabbit pAb (11744-1-AP) and acetylated tubulin mouse mAb (66200-1-IG) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) for IFT81, and Rhodamine-Goat anti-rabbit IGG for 66200-1-IG.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer using 11744-1-AP (IFT81 antibody) at dilution of 1:50 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-IFT81 (IP:11744-1-AP, 3ug; Detection:11744-1-AP 1:500) with mouse brain tissue lysate 7500ug.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-IFT81 (IP:11744-1-AP, 4 ug; Detection:11744-1-AP 1:500) with mouse brain tissue lysate 5200 ug.