Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- NB100-375 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-375, RRID:AB_10003335
- Product name
- Rabbit Polyclonal SMC4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Microcephalin/MCPH1 associates with the Condensin II complex to function in homologous recombination repair.
SYCP2 and SYCP3 are required for cohesin core integrity at diplotene but not for centromere cohesion at the first meiotic division.
Wood JL, Liang Y, Li K, Chen J
The Journal of biological chemistry 2008 Oct 24;283(43):29586-92
The Journal of biological chemistry 2008 Oct 24;283(43):29586-92
SYCP2 and SYCP3 are required for cohesin core integrity at diplotene but not for centromere cohesion at the first meiotic division.
Kouznetsova A, Novak I, Jessberger R, Höög C
Journal of cell science 2005 May 15;118(Pt 10):2271-8
Journal of cell science 2005 May 15;118(Pt 10):2271-8
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMC4 Antibody [NB100-375] - Whole cell lysate (90 mcg for WB; 0.5 mg for IP/WB) from 293T cells (lanes 1, 3 and 5), normal human lymphoblasts (lane 2) or SMC4 transfected 239T cells (lanes 4, 6 and 7) separated on an 8% SDS-PAGE gel. Affinity purified rabbit anti-SMC4 used at 0.2 or 0.1 mcg/ml for WB and 5 mcg for IP.