Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-86635 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-86635, RRID:AB_11054361
- Product name
- Rabbit Polyclonal SMC4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human SMC4 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references SMC5/6 is required for the formation of segregation-competent bivalent chromosomes during meiosis I in mouse oocytes.
Hwang G, Sun F, O'Brien M, Eppig JJ, Handel MA, Jordan PW
Development (Cambridge, England) 2017 May 1;144(9):1648-1660
Development (Cambridge, England) 2017 May 1;144(9):1648-1660
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMC4 Antibody [NBP1-86635] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-544
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMC4 Antibody [NBP1-86635] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SMC4 Antibody [NBP1-86635] - Staining of human kidney shows strong cytoplasmic positivity with granular pattern in tubule cells.