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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [16]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- A010-20 - Provider product page
- Provider
- Badrilla Ltd
- Proper citation
- Badrilla Cat#A010-20, RRID:AB_2631042
- Product name
- SERCA2a Rabbit Anti-Serum
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- SERCA2a is a Ca2+ pump located in the sarcoplasmic reticulum of cardiac and slow-twitch skeletal muscle. The enzyme transports Ca2+ from the cytoplasm into the SR using the hydrolysis of ATP as the energy source for this reaction. Two Ca2+ are transported for each ATP hydrolysed. This Ca2+ transport is the principal means of muscle relaxation in cardiac and slow-twitch skeletal muscle. The activity of SERCA2a is moderated by its interaction with phospholamban. SERCA2a is one isoform of SERCA2: this isoform differs from SERCA2b and SERCA2c at the extreme carboxy terminus only. Antibody A010-20 recognises the C-terminal sequence which distinguishes SERCA2a from SERCA2b and SERCA2c.
- Reactivity
- Human, Mouse, Rat, Canine, Rabbit
- Host
- Rabbit
- Antigen sequence
C-LEPAILE997- Isotype
- IgG
- Vial size
- 20 µl
- Storage
- Lyophilised: 4 °C Reconstituted:-80 °C
- Handling
- Reconstitute lyophilised powder in 20 µl of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freeze - thaw cycles
Submitted references A calcium-sensitive promoter construct for gene therapy.
Functional evidence for an active role of B-type natriuretic peptide in cardiac remodelling and pro-arrhythmogenicity.
Fructose diet treatment in mice induces fundamental disturbance of cardiomyocyte Ca2+ handling and myofilament responsiveness.
Fatty acid synthase modulates homeostatic responses to myocardial stress.
Carbon monoxide exposure enhances arrhythmia after cardiac stress: involvement of oxidative stress.
Nogo-A knockdown inhibits hypoxia/reoxygenation-induced activation of mitochondrial-dependent apoptosis in cardiomyocytes.
Aromatase deficiency confers paradoxical postischemic cardioprotection.
Conditional overexpression of neuronal nitric oxide synthase is cardioprotective in ischemia/reperfusion.
Moderate exercise prevents impaired Ca2+ handling in heart of CO-exposed rat: implication for sensitivity to ischemia-reperfusion.
Sarcolemmal Ca2+-ATPase ability to transport Ca2+ gradually diminishes after myocardial infarction in the rat.
Positive inotropic effects of epigallocatechin-3-gallate (EGCG) involve activation of Na+/H+ and Na+/Ca2+ exchangers.
Overexpression of TEAD-1 in transgenic mouse striated muscles produces a slower skeletal muscle contractile phenotype.
Myocardial sarcoplasmic reticulum Ca2+ ATPase function is increased by aerobic interval training.
Multiple alterations in Ca2+ handling determine the negative staircase in a cellular heart failure model.
Calsequestrin targeting to sarcoplasmic reticulum of skeletal muscle fibers.
Expression of slow skeletal troponin I in adult mouse heart helps to maintain the left ventricular systolic function during respiratory hypercapnia.
Merlet E, Lipskaia L, Marchand A, Hadri L, Mougenot N, Atassi F, Liang L, Hatem SN, Hajjar RJ, Lompré AM
Gene therapy 2012 Mar 29;
Gene therapy 2012 Mar 29;
Functional evidence for an active role of B-type natriuretic peptide in cardiac remodelling and pro-arrhythmogenicity.
Thireau J, Karam S, Fauconnier J, Roberge S, Cassan C, Cazorla O, Aimond F, Lacampagne A, Babuty D, Richard S
Cardiovascular research 2012 Jul 1;95(1):59-68
Cardiovascular research 2012 Jul 1;95(1):59-68
Fructose diet treatment in mice induces fundamental disturbance of cardiomyocyte Ca2+ handling and myofilament responsiveness.
Mellor KM, Wendt IR, Ritchie RH, Delbridge LM
American journal of physiology. Heart and circulatory physiology 2012 Feb 15;302(4):H964-72
American journal of physiology. Heart and circulatory physiology 2012 Feb 15;302(4):H964-72
Fatty acid synthase modulates homeostatic responses to myocardial stress.
Razani B, Zhang H, Schulze PC, Schilling JD, Verbsky J, Lodhi IJ, Topkara VK, Feng C, Coleman T, Kovacs A, Kelly DP, Saffitz JE, Dorn GW 2nd, Nichols CG, Semenkovich CF
The Journal of biological chemistry 2011 Sep 2;286(35):30949-30961
The Journal of biological chemistry 2011 Sep 2;286(35):30949-30961
Carbon monoxide exposure enhances arrhythmia after cardiac stress: involvement of oxidative stress.
André L, Gouzi F, Thireau J, Meyer G, Boissiere J, Delage M, Abdellaoui A, Feillet-Coudray C, Fouret G, Cristol JP, Lacampagne A, Obert P, Reboul C, Fauconnier J, Hayot M, Richard S, Cazorla O
Basic research in cardiology 2011 Nov;106(6):1235-46
Basic research in cardiology 2011 Nov;106(6):1235-46
Nogo-A knockdown inhibits hypoxia/reoxygenation-induced activation of mitochondrial-dependent apoptosis in cardiomyocytes.
Sarkey JP, Chu M, McShane M, Bovo E, Mou YA, Zima AV, de Tombe PP, Kartje GL, Martin JL
Journal of molecular and cellular cardiology 2011 Jun;50(6):1044-55
Journal of molecular and cellular cardiology 2011 Jun;50(6):1044-55
Aromatase deficiency confers paradoxical postischemic cardioprotection.
Bell JR, Mellor KM, Wollermann AC, Ip WT, Reichelt ME, Meachem SJ, Simpson ER, Delbridge LM
Endocrinology 2011 Dec;152(12):4937-47
Endocrinology 2011 Dec;152(12):4937-47
Conditional overexpression of neuronal nitric oxide synthase is cardioprotective in ischemia/reperfusion.
Burkard N, Williams T, Czolbe M, Blömer N, Panther F, Link M, Fraccarollo D, Widder JD, Hu K, Han H, Hofmann U, Frantz S, Nordbeck P, Bulla J, Schuh K, Ritter O
Circulation 2010 Oct 19;122(16):1588-603
Circulation 2010 Oct 19;122(16):1588-603
Moderate exercise prevents impaired Ca2+ handling in heart of CO-exposed rat: implication for sensitivity to ischemia-reperfusion.
Farah C, Meyer G, André L, Boissière J, Gayrard S, Cazorla O, Richard S, Boucher F, Tanguy S, Obert P, Reboul C
American journal of physiology. Heart and circulatory physiology 2010 Dec;299(6):H2076-81
American journal of physiology. Heart and circulatory physiology 2010 Dec;299(6):H2076-81
Sarcolemmal Ca2+-ATPase ability to transport Ca2+ gradually diminishes after myocardial infarction in the rat.
Mackiewicz U, Maczewski M, Konior A, Tellez JO, Nowis D, Dobrzynski H, Boyett MR, Lewartowski B
Cardiovascular research 2009 Feb 15;81(3):546-54
Cardiovascular research 2009 Feb 15;81(3):546-54
Positive inotropic effects of epigallocatechin-3-gallate (EGCG) involve activation of Na+/H+ and Na+/Ca2+ exchangers.
Lorenz M, Hellige N, Rieder P, Kinkel HT, Trimpert C, Staudt A, Felix SB, Baumann G, Stangl K, Stangl V
European journal of heart failure 2008 May;10(5):439-45
European journal of heart failure 2008 May;10(5):439-45
Overexpression of TEAD-1 in transgenic mouse striated muscles produces a slower skeletal muscle contractile phenotype.
Tsika RW, Schramm C, Simmer G, Fitzsimons DP, Moss RL, Ji J
The Journal of biological chemistry 2008 Dec 26;283(52):36154-67
The Journal of biological chemistry 2008 Dec 26;283(52):36154-67
Myocardial sarcoplasmic reticulum Ca2+ ATPase function is increased by aerobic interval training.
Kemi OJ, Ceci M, Condorelli G, Smith GL, Wisloff U
European journal of cardiovascular prevention and rehabilitation : official journal of the European Society of Cardiology, Working Groups on Epidemiology & Prevention and Cardiac Rehabilitation and Exercise Physiology 2008 Apr;15(2):145-8
European journal of cardiovascular prevention and rehabilitation : official journal of the European Society of Cardiology, Working Groups on Epidemiology & Prevention and Cardiac Rehabilitation and Exercise Physiology 2008 Apr;15(2):145-8
Multiple alterations in Ca2+ handling determine the negative staircase in a cellular heart failure model.
Palomeque J, Petroff MV, Sapia L, Gende OA, Mundiña-Weilenmann C, Mattiazzi A
Journal of cardiac failure 2007 Mar;13(2):143-54
Journal of cardiac failure 2007 Mar;13(2):143-54
Calsequestrin targeting to sarcoplasmic reticulum of skeletal muscle fibers.
Nori A, Valle G, Bortoloso E, Turcato F, Volpe P
American journal of physiology. Cell physiology 2006 Aug;291(2):C245-53
American journal of physiology. Cell physiology 2006 Aug;291(2):C245-53
Expression of slow skeletal troponin I in adult mouse heart helps to maintain the left ventricular systolic function during respiratory hypercapnia.
Urboniene D, Dias FA, Peña JR, Walker LA, Solaro RJ, Wolska BM
Circulation research 2005 Jul 8;97(1):70-7
Circulation research 2005 Jul 8;97(1):70-7
No comments: Submit comment
Supportive validation
- Submitted by
- Badrilla Ltd (provider)
- Main image
- Experimental details
- Western blot
- Sample type
- Canine sarcoplasmic reticulum
- Validation comment
- Ab stains for SERCA2a and is blocked by the immunizing peptide
- Primary Ab dilution
- 3.513888889
- Secondary Ab dilution
- 1.430555556
- Protocol
- Protocol
