A010-21AP

antibody from Badrilla Ltd
Targeting: ATP2A2 ATP2B, DAR, SERCA2
Provider product page for A010-21AP
Western blot
Immunohistochemistry

Antibody data

Product number
A010-21AP - Provider product page
Provider
Badrilla Ltd
Product name
Anti-SERCA Y/IF4 (AP) Mouse monoclonal IgG
Antibody type
Monoclonal
Antigen
Synthetic peptide
Description
SERCA1 is the Ca2+-pump (109kDa) located in the sarcoplasmic reticulum (SR) of fast-twitch skeletal muscle and SERCA2 is the Ca2+-pump located in the sarcoplasmic reticulum (SR) of cardiac, smooth and slow-twitch skeletal muscle. The enzyme transports 2 Ca2+ from the cytoplasm into the SR using the hydrolysis of 1 molecule of ATP to power the reaction. This terminates muscle contraction and promotes muscle relaxation. Mutations in SERCA1 are associated with Brody disease; mutations in SERCA2 are associated with Darier disease and down regulation of SERCA2 is seen in heart failure. The monoclonal antibody Y/1F4 was produced by immunizing mice with purified SERCA1: the antibody inhibits SERCA1 activity upon binding (Colyer et al., 1989). Antibody Y/1F4 recognises both SERCA1 and SERCA2.
Reactivity
Chicken/Avian
Host
Mouse
Antigen sequence
VGNKMFVKGA
Isotype
IgG
Vial size
50 µl
Storage
Lyophilised: 4 °C Reconstituted:-80 °C
Handling
Reconstitute lyophilised powder in 50 µl of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freeze - thaw cycles
ATP2A2 protein structure - A010-21AP shown in red.